Nuclease is a 5’-phosphodiesterase preparation produced from penicillium citrus strain, which is cultured and refined by the unique
fermentation process. Nucleases act on the phosphodiester bond between the 3’-carbon atoms of hydroxyl and phosphoric acid, which can hydrolyze RNA to 5'-nucleotides or flavoring nucleotides. As a quite important enzyme preparation, nuclease is often used to transform yeast extracts to natural enhancer and is widely used in the production of nucleotides.
Specification
Enzyme activity: Min.50000u/g
Features
·Yellow to brown powder;
·Optimum pH: 5.0; Stable pH range: 4.0~7.0;
·Optimum temperature: 70℃;
·Good solubility and thermal stability.
The following figures show the results of enzyme activity in the buffer solution system, which may be a little different from the actual situation when it is used. Please do preliminary test first and confirm the use conditions.
Application conditions
·Hydrolysis temperature: 60~70℃;
·Substrate concentration (RNA): 1%~5%, pH 4.0~7.0;
·Enzyme added amount: 1.2% (according to the substrate RNA);
·Hydrolysis time: 3~10 hours
Storage
Store in a cool and dry place, avoid exposure to sunlight.
