Ascorbic acid oxidase (Ascorbate oxidase, AO), referred to as: ASO, belongs to the family of ceruloplasmin, molecular weight is 140kD, composed of two identical subunits (70kD), the oxidation of ascorbic acid to dehydroascorbic acid, accompanied by oxygen to form water, and other redox reaction is coupled to the terminal oxidase role, oxidation can catalyze ascorbic acid, has anti-aging effect, has an important role in the metabolism of plants.
Aerobic oxidation of ascorbic acid oxidase catalyzes the formation of L- dehydroascorbic acid reaction.
EC1.10.3.3. Not to generate hydrogen peroxide. In higher plants (e.g., Cruciferae, cucumber, pumpkin) find and adrenal cortex.
Purification of the standard sample is blue and green protein, molecular weight of about 140000, with 0.46— 0.52% Cu (12 atom average 10— each enzyme molecule).
Also on D- Arabia sugar ascorbate, glucoascorbic acid. It can restore the polyphenol oxidase generated o- quinone, become part of plant respiration system. AO in the Cucurbitaceae (cucumber, pumpkin, gourd) content is higher, so the separation and purification of the enzyme is mainly extracted from these plants.
Our ascorbic acid oxidase (Ascorbate oxidase) extracted from fresh pumpkin peel, through filtration, concentration, sterilization, vacuum freeze drying process, is pure natural enzyme product safety and environmental protection.
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