Unit Definition: One unit will reduce the turbidity (A620) of a suspension of S. aureus cells from 0.250 to 0.125 in 10 minutes at pH 7.5 at 37 oC in a 6.0 mL reaction mixture.
Precautions and Disclaimer
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Preparation Instructions
This product is soluble in water (10 mg/mL), yielding a clear to slightly hazy solution. We recommended that fresh solutions be prepared, as the product loses activity in solution. We do not specifically recommend the freezing of lysostaphin solutions in aliquots. One published reference cites storage of 200 units/mL stock solutions of lysostaphin in a buffer of 0.05 M Tris-HCl and 0.145 M NaCl at pH 7.4.7 A second reference indicates storage of 2 mg/mL stock solutions in 20 mM sodium acetate buffer, pH 4.5, in frozen aliquots.8 However, we have not tested either situation ourselves.
Application
Degrade the bacteria which have a glycine peptide bond structure in the cell wall such as Staphylococcus aureus, to lyse the cell wall to achieve the purpose of sterilization.
Storage
At –20oC for 2 years storage time and at 2～8℃for 1 year.
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